PJB-2019-817
First In vivo Laxative, Anti-diarrheal, hepatoprotective and diuretic investigations of methanolic extract of Sterculia diversifolia in Mice
Fazle Rabbi
Abstract
Sterculia diversifolia is one of the most potent medicinal plant. The plant bears anti-bacterial, antifungal and anti-oxidant activities. We investigated the laxative, anti-diarrheal, hepatoprotective and diuretic activity of Sterculia diversifolia. The laxative activity was studied by counting wet stools while anti-diarrheal activity was performed by measuring gastrointestinal tract (GIT) motility. Hepatoprotective activity was studied by biochemical and histo-pathological analysis while diuretic activity was performed by urine collection protocol. In albino mice the dose of 30 and 100 mg/kg of crude MESD stem bark and leaves, significantly (p<0.05, p<0.001) produced wet feces while crude MESD stem bark at the dose of 30 and 100 mg/kg significantly (p<0.05, p<0.01) produced wet feces in subjects which were pretreated with atropine. Methanol extract of Sterculia diversifolia (MESD) stem bark and leaves increased fecal weight and water contents without producing diarrhea. The Constipation, indicated by decrease in fecal weight, was produced by atropine in mice. MESD stem bark and leaves dose-dependently (50 – 200 mg/kg) propelled charcoal meal through the small intestine of mice. The MESD stem bark and leaves at various doses (50, 100 and 200 mg/kg) significantly lowered various parameters of diarrhea. It produced shaped stools in mice. Pretreatment at doses of 150 and 300 mg/kg with MESD for 21 days, maintained the isoniazid and rifampicin-induced serum levels elevation of aspartate aminotransferase (p<0.001 and p<0.05), alanine aminotransferase (p<0.001), billirubin (p<0.001), alkaline phosphatase (p<0.001) and total proteins (p<0.001) as well as offered significant protection against histopathological changes in the liver. Diuretic activity was demonstrated by the administration of crude MESD stem bark and leaves of at 200, 300 and 400 mg/kg respectively. The Crude MESD stem bark and leaves extracts at a dose of 300 mg/kg (p<0.001) and 400 mg/kg (p<0.001) shows highly significant diuretic effect. The results show that MESD stem bark and leaves possess compounds which prevent constipation, diarrhea, hepatotoxicity and hypertension, which is different from the other agents. Hence it is concluded that Sterculia diversifolia stem bark and leaves bears laxative, antidiarrheal, hepatoprotective and diuretic effects.
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