PJB-2019-764
IDENTIFICATION AND PHYLOGENETIC ANALYSIS OF GLUTATHIONE PEROXIDASE-LIKE ENZYMES (GPXLs) IN WILD WATERMELON (CITRULLUS LANATUS)
Rameen Nadeem
Abstract
Among abiotic stresses, drought is the major stress that effect plants which in turn affects their working capability and productivity. The increased level of drought results in the production of high level of reactive oxygen species (ROS). Antioxidants are agents that scavenge the adverse effects of oxygen produced in the form of ROS. Under water deficit conditions in Citrullus lanatus, level of Glutathione peroxidase-like enzymes (GPXL’s) raises abruptly which hydrolyses H2O2 using Thioredoxin (Trx). C. lanatus is xerophyte and native of Kalahari Desert, Africa. In this study GPXLs in C.lanatus were analysed to exploit their role in water use efficiency. To validate the presence of GPXLs, a conserved motif (signature 3) of GPXLs was amplified using PCR in wild watermelon.
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