PJB-2019-730
Characterization of genetic diversity in Epimedium brevicornum using ISSR and CDDP markers
Yuanyuan Duan
Abstract
Epimedium brevicornum is a traditional and edible medicinal herb that is used to tonify kidneys and strengthen tendons and bones. In this study, two types of molecular markers, namely, inter simple sequence repeat (ISSR)and conserved DNA-derived polymorphism (CDDP) markers, were used to evaluate the genetic diversity of Epimedium brevicornum. The results showed that both ISSR and CDDP markers were useful for estimating genetic diversity. Forty-six polymorphic (86.79%) bands were generated by 7 random ISSR primers, while 82 polymorphic (95.35%) bands were generated by 11 CDDP primers. The genetic similarity coefficients of the ISSR primers ranged from 0.019 to 0.60, while those of the CDDP primers varied from 0.048 to 0.871. Moreover, the CDDP molecular marker technique was a preferable method for assisted selection of stress-resistant varieties. Two superior resistant germplasms (BQ2 and MPC2) were screened, which will enable the selection and breeding of stress-resistant varieties of Epimedium brevicornum.
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