PJB-2019-710
Performance of Aralia elata seedlings under the influence of ethyl methanesulfonate
Xihong yu
Abstract
This experiment was conducted to study the effect of ethyl methanesulfonate (EMS) treatment on growth and biomass of Aralia elata L seedlings. EMS was applied to the seedlings either by root-soaking or whole-plant spraying with different concentrations (v/v) i.e. 0 (control), EMS-19 μM, EMS-38 μM, and EMS-76 μM. Growth in terms of height was higher in the spraying treatment by 10% than root-soaking treatment. Compared to the control, the EMS-38 treatment resulted in lower cone-sting density (1.3 individuals/cm2) and the EMS-76 treatment resulted in lower fine root biomass (0.07 g/plant), length (215 cm), and tip-number (39.8). Through digital analysis on shoot image, EMS-treated seedlings had lower green-color index (41‒56) than controlled seedlings (61‒84). Color and brightness indices were positively correlated with fine root growth and biomass, while red-color index had a positive relationship with height growth. Therefore, it can be concluded that EMS at the rate of 38 μM can be used to induce less-stings mutants for A. elata seedlings, whose fine root growth can be predicted by digital analysis for shoot color.
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