PJB-2019-703
Antioxidant and Phytochemical composition of Leaves, Stem and Root Extracts of Withania coagulans and Withania somnifera.
Muhammad Farooq Azhar
Abstract
Different plant parts of Withania coagulans and Withania somnifera belonging to family Solanaceae were exploited for biochemical studies. These are wild plants and mostly considered as poisonous plants, although limitedly used in folk medicines in Pakistan. The extraction yield in menthol was also calculated for root, stem and leaves of both plants. The % of extract yield in leaves of W. coagulans was recorded high (7.6%) than stem (6.3%) and root (6.3%). The % extract yield of all parts of W. coagulans was greater than the same parts of W. somnifera. The pattern of % extract yield is similar for W. somnifera as in W. coagulans. In leaves its higher (5.5%) and a decreases from stem (5.2%) to roots (4.7%). Flavonoids and total phenolics were determined by using Folin-Ciocalteu reagent and aluminum chloride reagent through spectrophotometric assay. Maximum value of total phenolics and flavonoids were observed in W. coagulans leaves (58.21 mg GEA/g and 47 mg RE/g). There is a similar decreasing pattern in total phenolics and flavonoids contents from leaves to stem and root extracts. Antioxidant activities in different parts extracts of both plants were calculated at different levels. All parts of W. coagulans exhibits more antioxidant activity than W. somnifera. These results reveal the presence of useful biomedical components in both species which could be used in formulation of modern medicines.
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