PJB-2019-702
POLYMORPHIC STUDY OF MELIA AZEDARACH L. (CHINABERRY) WITH THE USE OF DIFFERENT MOLECULAR MARKERS
Nadir Ali Rind
Abstract
Abstract Genetic variation in the natural growing populations handles their potential to face challenging ecological and environmental conditions that leads to variation. Genetic distances among all populations of Chinaberry plants were analyzed by generating dendogram which shows phylogenetic relationship among them. Different conservation strategies should be applied to conserve the genome of the naturally growing M. azedarach (L) populations. Genomic DNA was extracted with the protocol followed by TM Plant genomic DNA Mini-prep Kit method. Total amount of DNA was quantified through Qubit® 2.0 Fluorometer. PCR based RAPD and SSR markers were applied for the study of genetic polymorphism among chinaberry DNA samples collected from three different areas of Sindh, Pakistan and five different locations of Turkey. Molecular markers were applied which showed polymorphic bands. According to Jaccard's similarity index with RAPD markers highest diversity was noted between Edirne and Izmir while the highest similarity index was noted between the genome of Istanbul and Adana. With the applications of SSR markers highest similarity index was noted among genomes of Edirne and Kocaeli. Key words: Genetic diversity, PCR, RAPD, SSR, Polymorphism.
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