PJB-2019-682
MOLECULAR ASSESSMENT OF ALOE SPECIES BY RAPD MARKERS
Huma Rao
Abstract
The purpose of this study was to analyse genetic variation between sixteen Aloe species. This leaf-succulent genus comprises a total of ±548 accepted species. Aloe gel is directly used to cure diabetes, face spots, pimples while, hair oil mixed with aloe jell also prevents hair fall. Furthermore, warmed pulp is applied for the treatment of rheumatism, swelling and paralysis. It is considered as a holy plant. The molecular marker technique; random amplified polymorphic DNA (RAPD) was performed. Aloe samples were obtained from DHA Karachi and Centre for plant conservation, University of Karachi. DNA were isolated using modified CTAB method. RAPD was performed to analyse diversity/similarity among sixteen Aloe species. Genetic diversity and phylogenetic analysis using nineteen RAPD primers showed 100% polymorphism. The pair wise similarity between analysed Aloe species ranged from 0.111 to 0.621. Dendrogram analysis using NTSYSpc software revealed that sixteen Aloe species can be divided into two main clusters and sub-clusters.
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