PJB-2019-658
GENETIC VARIETY ANALYSIS FOR BREEDING PURPOSES IN WILD AND CULTIVATED SUNFLOWER GERMPLASM (Helianthus annuus sp.)
Maissa Mohamed Morsi
Abstract
Molecular genetic methods show conclusive turn in phylogeny investigations and, therefore, understanding the allocation and extension of genetic diversity inside and among species. The current study aims to evaluate genetic diversity using molecular markers (5 SSR and 5 ISSR) on 13 sunflower (Helianthus annuus) genotypes from different regions. The data acquired with the highest degree of polymorphism were confirmed. Polymorphic bands (24 and 55) were demonstrated totally through SSR and ISSR, severally. Nevertheless, ISSR was superior in exhibiting polymorphism rate of (97.37%) than SSR markers (90.83%). A range from 2 to 21 of amplified fragments, with molecular weights varying from 34 to 1331bp. Genetic similarity coefficient was used to set UPGMA dendrogram. Dissimilarity was found in genotypes clustering within groups, whilst the manner of clustering in genotypes kept adjacent in ISSR and aggregated data of SSR and ISSR. The Dice similarity diverged between 0.058 and 0.792, 0.168 and 0.770, and 0.154 and 0.847 with SSR, ISSR and the combined phylogeny dendrogram, respectively. Genetic variance of sunflower accessions of wild and cultivated cultivars from different geographic regions was established. Knowledge of these results could be applied to confirm a wide genetic basis for outlook sunflower and manage germplasm breeding programs.
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