PJB-2019-591
PhPDS is involved in leaf and chloroplast development in petunia
Qian Wei
Abstract
Phytoene desaturase (PDS) is a rate-limiting enzyme in carotenoid biosynthesis and is usually used as marker gene in plant genetic transformation, because of the easily observed photoblenching phenotype. However, the function of PDS gene has been reported only in very few plants. In this study, petunia (Petunia hybrida) PhPDS gene was isolated. Phylogenetic tree of PDS of 27 plant species showed that PDSs are single copy gene in most plant species. Quantitative real-time PCR assays showed that PhPDS expression is spatio-temporally regulated. VIGS-mediated silencing of PhPDS resulted in albino leaves and sepals corresponding decreased contents of chlorophyll and carotenoids. Further micrographs showed that PhPDS silencing resulted in the increased number and size of palisade cells and spongy cells in leaves. The thylakoid in chloroplasts could not be formed in photobleaching leaves in PhPDS-silenced plants, and the chloroplasts could not be normally developed and some etioplasts were produced. These results suggest that the PhPDS plays an important role in leaf and chloroplast development.
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