PJB-2019-589
SALICYLIC ACID SUPPRESSES JASMONIC ACID- OR ETHYLENE-MEDIATED PROTECTION AGAINST SCLEROTINIA SCLEROTIOYUM IN BRASSICA NAPUS
ZHENG WANG
Abstract
Defense signaling responses mediated by phytohormones salicylic acid (SA), jasmonic acid (JA) and ethylene (ET) were widely studied in interactions of the model plant Arabidopsis thaliana and various pathogens. Rapeseed (Brassica napus) is an economically important oilseed crop. And Sclerotinia sclerotiorum, one of the most damaging pathogens of many economically important crops, causes a highly destructive disease in this crop resulting in significant economic losses. In the present study, the expression proï¬les of biosynthesis genes of the three phytohormones and their signaling marker genes were comparatively analyzed between the resistant Brassica napus cultivar Zhongshuang 9 and the susceptible one 84039 after this pathogen infection using quantitative real time-PCR (qRT-PCR). The results showed that these genes related to the three signaling pathways were differentially expressed in the resistance cultivar and the susceptible one, suggesting that the activation of both JA and ET signaling is more effective in the resistant cultivar than in the susceptible one in response to S. sclerotiorum infection while the activation of SA signaling is the opposite. Furthermore, the pharmacological experiments revealed that SA suppressed the JA and ET signaling, but the JA signaling and ET signaling promoted each other, and that SA application attenuated methyl jasmonate (MeJA)- or the ET precursor 1-aminocyclopropane-1-carboxylic acid (ACC)-conferred protection against S. sclerotiorum in B. napus. Our data provided an understanding about defense responses to the pathogen in B. napus, which will be of service to designing strategies to curb diseases caused by S. sclerotiorum in the economically important oilseed crop.
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