PJB-2019-562
IDENTIFICATION OF MULTIPLE BACTERIAL BLIGHT RESISTANT GENES AND FREGRENCE GENE IN RICE THROUGH STS MARKER
Ayesha Bibi
Abstract
Bacterial blight caused by Xanthomonas Oryzae pv. Oryzae (Xoo) is the most destructive disease of rice that limits rice yield in all major rice-growing regions of Pakistan, especially in irrigated lowland conditions. Since bacterial pathogen is difficult to manage, development of host plant resistance is the most effective mean to control this disease. For molecular characterization of these 96 accessions against bacterial blight and fragrance, tightly linked STS markers were used to amplify the fragment. Out of 96 accessions, 42 accessions were found to have Xa4 resistant gene, while 54 were lacking it, fifty-two accessions were found to have a xa5 resistant gene which is a most prevalent gene in Northern areas germplasm, while Xa21 resistant gene and fgr were absent in present germplasm. Identification of these genes in rice could be utilized for increasing the level of resistance of existing rice varieties
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