PJB-2019-545
GENOME-WIDE IDENTIFICATION AND CHARACTERIZATION OF THE HOG GENE FAMILY IN AGARICUS BISPORUS
Lihong Han
Abstract
The high-osmolarity glycerol (HOG) gene family, a member of MAPK super family, is demonstrated to be involved in various developmental and physiological processes of fungi. Despite the availability of recently released draft genome sequences of Agaricus bisporus (Morin et al., 2012), no comprehensive investigation of these family members has been performed. In this study, We identified 118 AbHOG genes from the genome of A. bisporus. Based on the phylogenetic relationship of their encoded proteins, AbHOG genes were classified into four groups. The analysis of gene structure showed AbHOG genes contained many short and abundant introns, which indicated that exon loss and gain occurred frequently during evolution. Ten conserved motifs were identified in HOG proteins, which revealed the functional conservativeness and diversity of AbHOG family. Transcriptome expression profiling indicated that 116 AbHOG genes were expressed differentially in the six developmental stages, most of them exhibited high expression levels. Several important hormone-related and biotic and abiotic stresses-related cis-acting elements were found in the promoters of the AbHOG genes. An interaction network of AbHOG associated with Saccharomyces cerevisiae orthologs was constructed, suggesting AbHOG proteins play crucial roles in many fundamental mechanisms by regulating downstream factors or being regulated by upstream genes. These results revealed that AbHOG family may have great effect on morphogenesis and multi-stress tolerance. The genome-wide identification, evolutionary, gene structure and expression analyses of AbHOG genes provide a comprehensive overview of their potential involvements in development and stress responses, and new opportunities for cloning and functional studies of stress tolerance in fungi.
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