PJB-2019-457
SUBMERGED CULTIVATION OF MEDICINAL MASHROOM IN HYDROLYSATE OF LIGNO-CELLULOSIC MATERIAL.
Shamaila Kausar
Abstract
Ganoderma lucidum belongs to the family Ganodermataceae and found in Japan, China and some other parts of Asia. Traditionally it is used against diabetic, cancer prevention agent, antitumor, immunomodulation, antimicrobial and antiviral activities. Due to difficulty in field cultivation submerged fermentation was employed as a promising method for efficient and large-scale production of mycelia biomass and bioactive metabolites. Cellulose was used in the form of lignocellulosic substrate. The Ganoderma lucidum which is medicinal and edible mushrooms was successfully grown in the form of mycelial biomass in static submerged culture in petri plates and flasks. The present study is based on the utilization of hydrolyzates of lignocellulosic materials such as Peanut cort, Sugarcane bagasse and Wheat Straw were used after hydrolysis. A Static Fermentation Technique was employed to investigate the mycelial growth, instead of Fruiting Body. Ganoderma lucidum was kept up on PDA (potato dextrose agar) medium in petri dishes at 4℃ and brooded at 25 ◦C for 5 days for the development of G. lucidum and generation of Ganoderic Acid. Morphology of G. lucidum on various Hydrolysates was white and delicate like cotton unpredictable shape, Cloud like appearance spread in general plate and multiple little sporadic white cotton like shape with string like projections. We got a Ganoderic Acid from the Hydrolysates of Peanut cort concentrate, Sugarcane bagasse concentrate and Wheat straw concentrate at a concentration of 0.006g/L, 0.011g/L, and 0.017g/L respectively.
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