PJB-2019-420
Effect of biogenic silver nanoparticles on the growth parameters of Begomo virus inoculated Cumumis sativa and Abelmoschus esculentus plants
Arifa Tahir
Abstract
The present study is an attempt to observe the antiviral activity of silver nanoparticles against Begomo virus on Cumumis Sativa (cucumber) and Abelmoschus Esculentus (Okra). The two plant species, Cucumis sativus and Abelmoschus esculentus were treated with AgNPs at the germination stage.After twenty days of germination , the inoculum of Tomato Leaf Curl New Delhi Virus (ToLCNDV) was infiltrated on the lower side of younger leaves between the veins. Different parameters including physical (number of leaves, root length, plant height), bio-physical (fresh, turgid and dry weight of leaves, relative water content and water deficit) and biochemical (chlorophyll and sugar content) were studied to assess the effect of AgNPs on viral activity. Semi-quantitative PCR technique was applied to analyze the virus titer by using coat protein primer. The results showed a very little effect on the growth of roots of both plants but no significant change was observed in the root and shoot length. The chlorophyll a &b content was also reduced in treated sample of both plants (Cucumis sativus and Abelmoschus esculentus) as compared to control. The appearance of light band in PCR analysis depicted reduction of viral titer which in turn presented viral suppressant activity of silver nanoparticles. The future of agricultural crops will be secured by using nanotechnology for the protection of crops against viral diseases.
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