PJB-2019-393
Effects of genotype, plant regulators and sucrose on callus induction and cell suspension cultures of Iranian rice (Oryza sativa L.)
Mansour Farkhondeh tale navi
Abstract
Abstract: An efficient protocol for in vitro callus induction and cell suspension culture of Rice has been established using mature embryo. In this way the in the first experiment effect of different concentrations of 2, 4-dichlorophenoxy acetic acid (0.0, 1.0, 1.5, and 2.0 mg L-1) and genotype were investigated. There was significant difference between cultivars in response to callus production. Among evaluated genotypes, Hashemi produced the highest (weight 374.33 mg) and Gohar produced lowest (weight 106.33 mg) callus biomass. Four cultivars selected for next experiment according to their callus weight value. In the second experiment effect of different concentrations of kinetin (0.0, 1.0, and 2.0 mgL-1) and sucrose (30.0 and 60.0 gL-1) were tested for improvement callus quantity and quality for initiation of cell suspension cultures from friable calli. Significant difference in cells growth quantity was observed among the cultivars as well as among the kinetin and sucrose concentrations. The MS medium supplemented with 0.5 mgL-1 2,4-D, 2.0 mgL-1 kinetin, and 60.0 gL-1 sucrose exhibited the maximum cells growth for all genotypes. The Hashemi produced highest fresh cells biomass (3.97 g) and Kazemi produced lowest fresh cells (2.71 g). The growth curves of cell cultures revealed a sigmoidal-type pattern and high cell viability continued for about 4 days and then the declined as a result of a lack of a carbon source.
Keywords: Callus induction, Cell suspension, Cell viability, Plant growth regulators, Rice
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