PJB-2019-345
Exopolysaccharide (EPS) composition analysis of Bacillus cereus (EG4) associated with Lumbricus terrestris at different Physiological parameters
sana yousaf
Abstract
Bacteria live in association with living and non-living objects in the form of Biofilm. Earthworms are the engineers of soil as they work along with microbes in synergism that helps to improve soil quality, water holding capacity and soil aggregation and improves plant growth. Bacillus cereus (EG4 accession NO: KY435707) previously isolated from external surface of earthworms, were used for exopolysaccharides (EPS) production and its characterization at different physiological parameters, growth media (LB-broth and M9-broth) and agitation etc. Qualitative analysis of EPS was done by using congo-red supplemented BHI agar medium and EPS forming colonies were determined on the basis of their dark brown color. Quantitative analysis of EPS was done in terms of glucose and protein content. In general, it was observed that the Bacillus cereus produce more EPS (mg/ml of media) at non-shaking conditions at 37â°C. Protein contents of EPS were higher in LB-broth at non-shaking conditions while glucose contents were higher in M9-broth at 37â°C under shaking conditions. Plant growth promoting potential of the EPS producing strains showed significant improvement in seedling length at 100 mM salt stress as compared to its respective control conditions. Microscopy of seedling sections stained with sudan III revealed the presence of lipid in endodermis layer. Present findings showed the utilization of earthworm associated microorganisms as plant growth promoter under salinity.
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