PJB-2019-295
PHOTOCHEMICAL , MEDICINAL INVESTIGATION AND APPLICATIONS OF WITHANIA COAGULANS(DUNAL) FRUITS
Muneeb ur Rehman
Abstract
Withaniacoagulans plays a significant traditional role to treat a large number of human diseases. The present study aimed at determining phytochemical screening, antibacterial, antifungal and antioxidant activities of W. coagulans. :The study was carried out in Dera Ismail Khan KPK Pakistan in 2017For plant collection, identification, preparation and fractionation of crude extract and preparation of culture media standard procedures were followed. Alkaloid, sugar, and terpanoid were present in all fractions. Flavonoid was present in methanolic and aqueous extract. Saponin was present in ethyl acetate, benzene, n-hexane and chloroform... Disc diffusion method was applied to check antibacterial activities of all the extracts against 5 bacterial strains using Moxifloxacin as reference. Highest antibacterial activity was shown by methanolic fruit extract against Proteus vulgaris giving 15.5mm zone of inhibition. The n-hexane showed minimum antibacterial activity at 500μg/ml concentration giving 4.5mm against B. subtilis followed by 5.0mm against S. aureus, The aqueous extract gave most conspicuous zone (24.1mm) against A. niger. N-hexxane and benzene showed minimum inhibitory activities against all strains. Ethyl acetate and chloroform gave moderate antifungal activities. Antioxidant activity ascended with increasing concentration of all the extract however;.
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