PJB-2019-290
INFESTATION, DNA BARCODING AND PHYLOGENETIC ANALYSIS AND OF YELLOW PEACH MOTH, CONOGETHES PUNCTIFERALIS (GUENEE) (LEPIDOPTERA: CRAMBIDAE) INFESTING GUAVA IN PUNJAB, PAKISTAN
jam nazeer Ahmad
Abstract
Yellow peach moth, Conogethes punctiferalis Guenée (Lepidoptera: Crambidae) is an important pest of some fruit crops and vegetables worldwide. Conogethes and Dichocrocis have been considered synonyms in the older literature; and punctiferalis is species complex and often seen combined with Dichocrocis. Recently, insect pest infested guava fruits were collected from Guava orchards in Punjab, Pakistan and placed in plastic jars for adult emergence. Morphological study of Adults under microscope showed the similar pattern of black spots on body as reported by taxonomist for C. punctiferalis. Molecular identification and phylogenetic analysis was also done through PCR, Sequencing and Software (MEGA6). Mitochondrial cytochrome oxidase I (COI) gene based primer (LCO-1490/ HCO-2198) was used for DNA amplification and sequencing. From all collected C. punctiferalis samples, the PCR bands of 710bp were obtained in gel electrophoresis. The sequence analysis with sequences submitted at NCBI database (Accession number MK301225.1) revealed that C. punctiferalis has 99-100% similarity with C. punctiferalis sequences. This is the first report of infestation, identification of yellow peach moth, C. punctiferalis infesting Guava fruit from Punjab, Pakistan.
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