PJB-2018-1015
INDUCTION OF SOMATIC EMBRYOGENESIS FROM CARICA PAPAYA L. ‘EKSOTIKA’
BAKER AL-SHARA
Abstract
Factors affecting induction of somatic embryogenesis in Carica papaya L. cv. ‘Eksotika’ were evaluated. Experiments were carried out to examine the effect of medium type, boric acid concentration, and 2,4-dichlorophenoxyacetic acid (2,4-D) concentration on somatic embryo induction from immature zygotic embryos. The highest average callus weight (0.088 g) and highest average callus diameter (0.79 cm) after 6 weeks were obtained in ½ MS medium supplemented with 7.5 mg/L 2,4-D. Although the highest percentage of somatic embryo formation (66.1%) was observed in McCown Woody Plant Medium supplemented with 2.5 mg/L 2,4-D, the average callus weight was low (0.051 g) and the callus was mucilaginous and compact with only a small friable portion. In the boric acid experiment, the highest percentage of somatic embryo formation (68.7%) was observed when 54 mg/L boric acid was added to ½ MS medium with full-strength vitamins.
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