PJB-2018-951
ASSESSMENT OF GENETIC DIVERSITY IN SOME RICE (ORYZA SATIVA L.) GENOTYPES USING AGRO-MORPHOLOGICAL TRAITS AND INTRON-EXON SPLICE JUNCTIONS (ISJ) MARKERS
Ebrahim Abdelsalam Ramadan
Abstract
Fourteen superior Egyptian rice (Oryza sativa L.) genotypes were evaluated under Egyptian conditions during seasons 2015/16 and 2016/17 to assess genetic diversity using seven agro-morphological traits and seven intron-exon splice junctions (ISJ) markers. Field performance results exhibited that the studied genotypes exhibited a large amount of variation for their studied traits. Cluster analysis based on Euclidean coefficient of dissimilarity classified the studied genotypes into two major clusters at 42% dissimilarity coefficient. The results of PCA indicated that the first three principal components revealed the highest Eigen values and explained cumulative variation of 81.04% of the total variation among the studied traits. Results of ISJ markers revealed high polymorphic ratio ranged from 30% for to 85.71% with an average of 58.9%. About 28 highly informative polymorphic bands were generated, among them 15 bands were related to the subspecies. Polymorphic information content (PIC) values ranged from 0.289 for ISJ-9 to 0.480 for ISJ-1 with an average of 0.375. The coefficient of similarities based on semi-random data among the studied genotypes ranged from 0.53 to 0.9 with an average of 0.66. All genotypes clearly grouped into two major clusters in the dendrogram at 58% similarity based on Jaccard’s similarity index. The first cluster represented the Indica and Indica/Japonica rice genotypes, while the second cluster represented the Japonica genotypes. These results indicated that agro-morphological traits and ISJ markers may be an efficient tool for varietal identification and assessing genetic diversity in rice. The results highlight the existing diversity among the studied genotypes and hence their potential use in breeding programs. The simplicity and reproducibility of ISJ markers indicates the potential utilization for molecular characterization, identification and purity assessment of rice genotypes.
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