PJB-2018-940
Paraffin Sectioning Technique for Sectioning of Siberian Wildryegrass (Elymus sibiricus L.)
De Ying
Abstract
Siberian wildryegrass (Elymus sibiricus L.) is an important type of Gramineae forage with high resistance, adaptability, and feeding value; accordingly, this organism plays an important role in vegetation restoration and ecological construction and should be studied in detail, particularly at the microscopic level. Paraffin sectioning is commonly used not only to study growth and structure of plant organs but also to perform histochemistry and conduct RNA in situ hybridization, tissue biopsy for in situ PCR, and so on, ever-expanding applications. Here, we described the details of paraffin sectioning to produce microsections of E. sibiricus; for every step, we described the specific details of the experimental method, including drug concentrations and treatment times, and briefly introduce the embryonic development of E. sibiricus. The aim of this report was to establish a foundation for further anatomical studies of Elymus and its relatives.
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