PJB-2018-862
PHYTOCHEMICAL ASSESSMENT OF PUNICA GRANATUM PEEL EXTRACTS AND THEIR ANTIMICROBIAL ACTIVITIES AGAINST MULTI DRUG RESISTANT ORGANISMS
ZIA UR RAHMAN
Abstract
The present research work was conducted to evaluate the phytochemical analysis of Punica granatum peel extract and their antimicrobial activities against multidrug resistant bacterial and fungal isolates. The result of phytochemical screening of methanol, ethyl acetate and hot water extracts of Punica granatum showed the presence of flavonoids, alkaloids, saponins, glycosides, terpenoids, phenols and tannins which was further confirmed by FTIR analysis. A total of fourteen bacterial and eight fungal isolates were used in the present study which were previously identified in different laboratories of KP. Disc diffusion assay was performed to evaluate sensitivity of different antibiotics and antifungal agents. While agar well diffusion assay was carried out to determine the antibacterial and antifungal activities of different solvents peel extracts of Punica granatum. Antimicrobial sensitivity showed that Amoxicillin+ clavulanic acid was the most effective antibiotic with zone of inhibition 32 mm followed by Levofloxacin 29 mm, Ciprofloxacin 31 mm and Cefradine 29 mm against almost all tested bacterial isolates. Antifungal sensitivity showed that Voriconazole as compared to Nystatin was the most effective and produce the largest ZI (25 mm) against Penicillium. The ethyl acetate, methanol and hot water Punica granatum peel extract showed 100% antifungal activities against all fungal isolates and 90-98% activities against bacterial isolates. Furthermore, it was concluded from the results that the peel extract of Punica granatum had potent antibacterial and antifungal activities against MDR strains so, it can be used as alternative therapy against bacterial and fungal infections.
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