PJB-2018-822
INTER SPECIFIC GENETIC DIVERSITY WITHIN THE ETHNOMEDICINALLY IMPORTANT IPOMOEA L. SPECIES THROUGH MORPHOLOGICAL AND BIOCHEMICAL PROFILING
Noor Muhammad
Abstract
The present study has attempted to highlight the medicinal importance as well as genetic relationships among of Ipomoea species growing in Swat Valley, KP, Pakistan. There was a general agreement among the informers regarding the medicinal uses of plants and Informants Consensus Factor (ICF) value ranged from 0.991 to 0.965. The study shown that most of the informants agreed in the application of I. hederacea to use as blood purifier (ICF 0.991) that exhibited the highest fidelity level (100%). The intra and inter specific dissimilarity among the 28 genotypes of Ipomoea species viz., 14 genotypes of I. hedracea, 14 of I. purpurea, were tested using morphometric and biochemical profiling. Twenty four morphological characters were counted for the assessment of intra and inter genetic disagreement through traits similarity index and cluster analysis. Total seed protein profiling was carried out on 12% slab gel electrophoresis; 6 reproducible bands were detected in both I. Purpurea and I hedracea with molecular weight ranging from 10KDa to 180KDa. Intra locus contribution toward the genetic disagreement was 33.33% in I. hedracea and 66.66% in I. purpurea. In the same way, inter species locus contribution toward genetic diversity was 83.33%. Interestingly, locus 2 (L-2) was monomorphic in the collected germplasms and may be shown as generic specific locus for Ipomoea species. SDS-PAGE profiling based on two-way cluster plotting successfully determined the two species into separate clusters and the diversity found between and within Ipomoea species may offer unique avenues for novel drug discovery.
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