PJB-2018-819
Construction of Genetic linkage map and Identification of QTLs related to Agronomic traits in DH population of Maize (Zea mays L.) using SSR markers
JAE-KEUN CHOI
Abstract
Double haploid (DH) technology offers an array of advantages in maize genetics and breeding. In our study, we constructed a maize genetic linkage map using SSR markers and a DH population derived from a cross of normal corn (HF1) and normal corn (11S6169). A total of 200 SSR markers were assigned to 10 linkage groups that spanned 1,145.4 cM with an average genetic distance between markers of 5.7 cM. 68 SSR markers showed Mendelian segregation ratios in the DH population at a 5% significance threshold. A total of 15 quantitative trait loci (QTLs) for plant height (PH), ear height (EH), ear height ratio (ER), leaf length (LL), ear length (EL), set ear length (SEL), set ear ratio (SER), ear width (EW), 100 kernel weight (100KW), and cob color (CC) were found in the 121 lines in the DH population. These QTLs were mapped to chromosomes (ch.) 1, 2, 3, 4, 5, 7, and 10. Among these QTLs, two QTLs were associated with PH on ch. 4 and 10, one with EH on ch. 10, one with ER on ch. 5, two with LL on ch. 2 and 7, two with EL on ch. 2 and 5, two with SEL on ch. 7 and 10, two with SER on ch. 2 and 4, one with EW on ch. 3, one with 100KW on ch. 7, and one with CC on ch. 1. We found that four QTLs (qEL5, qEH10, qEW3, qCC1) were major QTLs accounting for over 15% of phenotypic variation. These new QTLs identified by this study may be suitable as molecular markers for selecting important agronomic traits in maize. The results of this study may help to improve the detection and characterization of agronomic traits and provide great opportunities for maize breeders and researchers using a DH population in maize breeding programs.
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