PJB-2018-792
Efficient protocol for micropropagation of Centella asiatica (L.) and essential oil profiling of micropropagated plant
Saeedeh Ghadirir Sardrood
Abstract
Centella asiatica (L.) is a herb of the Apiaciaea family that is very popular for its culinary and medicinal use. In recent years, due to overexploitation, and ineffectual efforts to revitalize this valuable herbal source, the abundance of C asiatica (L.) in nature has suffered significantly, increasing the risk of its extinction. Therefore, interest in micropropagation of this valuable herb through in vitro plant tissue culture techniques has grown considerably. In this study, mass production of C. asiatica (L.) was carried out through a cost effective micropropagation protocol and the chemical content of essential oils from micropropagated plants were analysed by Gas chromatography (GC) and Gas chromatography–mass spectrometry (GCMS) and compared with those of wild plants. After sterilization and establishment of nodal explants, the effects of 9 treatments of modified Murashige & Skoog (MS) medium were studied on the proliferation coefficient and shoot length. MS medium supplemented with 6-Benzylaminopurine (BA) 0.5 mgL-1, 6-(γ,γ-dimethylallylamino)purine (2ip) 0.5 mgL-1 and 1-Naphthaleneacetic acid (NAA) 0.1 mgL-1 was selected as the best treatment. In the comparison of the effect of 11 different hormonal treatments in MS medium on rooting percentage and acclimatization, the MS medium supplemented with Indole-3-butyric acid (IBA) 0.5 mgL-1 was chosen as the best treatment. In total, 34 and 45 compounds were identified and quantified in wild and micropropagated plants, representing 97.5% and 97.7% of the total oil, respectively. Also the oils were dominated by oxygenated sesquiterpenes, 70.8% and 42.6% in the oils from wild and micropropagated plants, respectively.
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