PJB-2018-787
PHENOTYPIC AND MOLECULAR CHARACTERISTICS OF IMPORTANT MESOPHILIC RHIZOBACTERIA ISOLATED FROM THE RHIZOSPHERE OF BT AND NON-BT COTTON CULTIVARS
Muhammad Ibrahim
Abstract
Rhizobacteria actively colonize the rhizosphere or soil surrounding the plant roots forming a mutualistic and beneficial relation with plants. The current study was conducted to isolate and characterizedthe rhizobacteria from the rhizosphere ofBt and non-Bt cotton cultivars, grown atfour sites of Multan district, Punjab, Pakistan.The rhizosphere of non-Bt cotton reportedan increased number of bacterial colonies (CFU/g) from thesoil on serially dilution agar plates than Btcotton,indicating the adverse effects of Bt toxinon soil health and bacterial flora. The bacterial strains were identified by detailed morphological and biochemical methods. For the confirmation of bacterial strain, 16S rRNA gene sequence analysis was carried out, that revealedthat all the isolates showed97 to 99% similarity with their nearest relatives through BLAST analysis.Bacillus cereus, B. subtilis, B. safensis, B. stratosphericus, B. pseudomycoidesand Serratia marcescens were identified in both Bt and non-Bt cotton cultivars. However, Pseudomonas monteilii, B. vietnamiensis, B. drentensis, B. licheniformis, B. endophyticus,and Lysinibacillus macroideswere presentin Bt cottonrhizosphere, whereas, B. toyonensis, B. flexus,andP. plecoglossicidawere present in non-Bt cotton cultivars. B. cereus, B. subtilis, and S. marcescenswere predominant speciesin the rhizosphere under study area.The results revealed that the rhizosphere of study areaisan affluent source of Bacillus species.
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