PJB-2018-776
TRANSCRIPTOME ANALYSIS OF LEAF OF BROUSSONETIA PAPYRIFERA INVOLVED IN RESPONSE TO CADMIUM STRESS
WAN ZHANG
Abstract
Cadmium (Cd) has a high degree of plant solubility and absorption capacity among all heavy metals and is considered to be the most toxic which can cause significant harm to humans through the food chain. Broussonetia papyrifera is a kind of economical tree species with higher comprehensive efficiency, which can be used as an excellent heavy metal adsorbent. However, the mechanism of Cd resistance of B. papyrifera from the perspective of molecular ecology is not mature enough. In order to elucidate the molecular regulation mechanism and key genes of B. papyrifera under Cd stress conditions. We performed transcriptome sequencing and data analysis on the leaf tissue of B. papyrifera exposed to Cd based on the Illumina Hiseq-PE150 platform. By annotating different databases, it was found that the functional classification of B. papyrifera was similar to that of most plants, possibly due to the fact that the protein sequence of each specific domain is conserved. We predicted 70,865 SSRs from the unigene library of B. papyrifera. A large number of transcription factor (TF) families such as Orphans, C2H2, C3H, MYB, bZIP, WRKY, AP2-EREBP, and bHLH were found to be involved in the stress response of heavy metals to Cd. A total of 345 differentially expressed genes (DEGs) were detected in both the control and experimental groups. KEGG enrichment analysis of DEGs revealed that the up-regulated genes were mainly involved in the "Linoleic acid metabolism" metabolic pathway, and down-regulated genes mainly controlled the "Photosynthesis" of B. papyrifera. And the genes involved in regulation in the two pathways were LOX1_5, LOX2S and psbA, psaE, respectively. Our research would provide genomic resources for genetic analysis of B. papyrifera; what¡¯s more, it could provide resources and basis for the screening of B. papyrifera resistance genes, as well as further genetic engineering studies and mine rehabilitation.
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