PJB-2018-755
Antioxidant, anti-inflammatory and analgesic activities of in vitro and field grown Iceberg lettuce extracts in rats
IQRA YOUNUS
Abstract
Iceberg lettuce is a nutrient rich edible crop containing vitamin B6, thiamin and folate. The increasing demand of natural products is moving the focus towards in vitro plant sources as factories for producing important secondary metabolites. The current study compares the biological activities of different solvent-derived extracts of field grown leaves and in vitro propagated Iceberg lettuce. The extracts were made with methanol, n-hexane, ethyl acetate and water for field-grown as well as in vitro cultured Iceberg lettuce plants and evaluated for their medicinal potential both in vitro and in vivo in rats. Total flavonoid and phenolic contents of plant extracts showed strong positive correlation with their antioxidant activities. The highest contents of flavonoids and phenolics were present in the methanolic extract of field grown leaves. The antioxidant, anti-inflammatory and analgesic activities of examined samples in rats were observed as: field-grown leaves extracts > callus extracts > regenerated shoots extracts. However, all of these activities in the extracts of callus culture were comparable to that of field grown plant extracts. The present study elucidates the significant and comparable activities of in vitro propagated callus culture of Iceberg lettuce, which can act as an alternate source of bioactive secondary metabolites.
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