PJB-2018-746
FUNGAL DIVERSITY OF THE MARBLE WASTE POLLUTED ECOSYSTEM IN THE PHYLOGENETIC PERSPECTIVES
Zeeshan Ahmad
Abstract
Phylogeny attributes to the evolutionary history of organisms. This study evaluated the isolation, characterization, molecular identification and phylogeny of micro fungal diversity collected from marble waste polluted ecosystem. A total of 15 factories at a distance of 3- 4 Km were randomly selected during in month of July - August, 2016. Overall 45 samples were collected i.e., one each from vicinity, 50 and 100 m distance of marble waste water effluent for each industry. Malt extract agar media was used for fungus growth and isolation of pure colonies. The obtained colonies were observed via digital colony observer. All the fungal isolates were characterized via Lacto-phenol cotton blue stain for their anatomical examination. The hyphae length and width of fungi were measured in micro meter (µm) using Piximeter software. Nuclear ribosomal Internal Transcribed Spacer (ITS) was amplified using ITS1-F and ITS4-R universal primers. ITS sequences were compared via BLAST network service of National Center for Biotechnology Information (NCBI) to affirm identification of micro fungus species. The preliminary results identified a total of six pure micro fungal isolates. Their molecular identification and phylogeny resulted Aspergillus brasilensi, Aspergillus sydowii, Aureobasidium leucosper, Fusarium petroliphilum, Curvularia aeria and alternaria alternata fungal species from marble wastewater polluted ecosystem. Morphologically most of these strains comprehended aseptate hyphae and black, brown, green, white to dark green colors. Whereas, anatomically these strains ranges from cylindrical to round, hyaline in lacto-phenol blue, thick to thin walled, smooth to ornamented surface with sharp scale and fusoid to ellipsoid in shape. These microbial strains will provide a frame work for future investigation with special reverences to phytoremediation of marble waste polluted ecosystem.
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