PJB-2018-745
MOLECULAR ASSESSMENT OF GENETIC VARIATION IN A COLLECTION OF LENTILS USING SEQUENCE-RELATED AMPLIFIED POLYMORPHISM MARKERS
NABIL A. MOHAMMED
Abstract
A collection of lentil landraces, mostly from Yemen (20 genotypes), but including landraces from Saudi Arabia (7), Egypt (4), Bangladesh (3), and two genotypes from the International Center for Research in the Dry Areas (ICARDA), was evaluated using twenty-one sequence-related amplified polymorphism (SRAP) primer combinations. A large number of useful polymorphic amplified fragments (796 out of 801) were detected, revealing a high degree of variation. The polymorphic information content (PIC) ranged from 91.7 to 97.5 and the discrimination power (DP) ranged from 2.14 to 7.79. The UPGMA trees, based on Jaccard similarity index matrices, classified the genotypes into four main clusters according to their geographical origin. This study highlights the extensive genetic diversity among the investigated lentil genotypes and shows the effectiveness of the SRAP technique in the study of the genetic variability of lentils, and possibly for substantiating the genetic peculiarity of ecotypes applying to obtain origin and quality marks.
To Cite this article:
Download
