PJB-2018-735
RESPONSES OF DETOXIFICATION ENZYMES AND GENES TO NICOSULFURON STRESS IN TWO GENOTYPES OF SWEET CORN DIFFERING IN NICOSULFURON TOLERANCE
JIAN WANG
Abstract
Accumulation of nicosulfuron will increase toxicity and induce the production of oxidative stress in plants. Acetolactate synthase (ALS, EC 2.2.1.6) and glutathione transferases (GSTs, E.C.2.5.1.18) represent important groups of cytoprotective enzymes participating in detoxification of xenobiotics and limiting oxidative damages of cellular macromolecules. The objective of this study was to investigate the toxic effect of nicosulfuron on the detoxification enzyme activities and gene expressions in maize seedling. Nicosulfuron-tolerant sweet inbred line ‘HK301’ and nicosulfuron-sensitive sweet inbred line ‘HK320’ were subjected to 80mg kg–1 nicosulfuron treatment when the fourth leaves were fully developed and the obtained effects were compared to water treatment as control. After nicosulfuron treatment, ALS and GST enzymes activities of HK320 were significantly lower than those of HK301. Compared to HK320, nicosulfuron treatment increased the transcript levels of GST1, ALS1 and ALS2 genes in HK301. These results suggest that on a transcription level, and in accordance with the responses of detoxifying enzymes play a vital role in reducing the toxicity of nicosulfuron and the oxidative stress induced by nicosulfuron of maize seedlings.
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