PJB-2018-701
Comparative proteomics analysis to identify differential proteins between self-compatibility and self-incompatibility in Citrus reticulata Blanco
Yi Ren
Abstract
Self-incompatibility (SI) is widespread mechanism of genetically controlled mate selection in most flowering plant. In Citrus, SI is one of the important factors resulting in seedless fruits. ‘Wuzishatangju’ (Citrus reticulata Blanco) is a natural bud sport derived from a self-compatibility (SC) cultivar ‘Shatangju’ and displays gametophytic self-incompatibility (GSI). However, its SI molecular basis is still unclear. In this study, we compared the protein profiles of pollen and pistil between ‘Wuzishatangju’ and ‘Shatangju’ using a gel-free quantitative proteomic analysis by tandem mass tag (TMT) technology. A total of 4506 proteins and 67 differential abundance proteins (DAPs) in pollen of the two cultivars were detected. Bioinformatic analyses revealed that 35 Gene Ontology (GO) functional groups were identified and 30 metabolic pathways were mapped in KEGG database. In pistil, a total of 5,462 proteins and 102 DAPs were obtained. 32 GO functional groups were identified and 51 metabolic pathways were mapped in KEGG database. In pollen, γ-aminobutyric acid (GABA) transaminase which can regulate the homeostasis of GABA and polyamine oxidase involved in polyamine metabolism were obtained and they were up-accumulation in ‘Wuzishatangju’ compared with ‘Shatangju’. This results indicated that the homeostasis of GABA and polyamine metabolism potentially were involved in SI reaction in ‘Wuzishatangju’. These results might facilitate our understanding of the molecular mechanisms of SI in Citrus.
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