PJB-2018-680
Micropropagation and influence of hormones on shoots morphology regenerated from callus culture of Chenopodium ambrosoides.
Tour Jan
Abstract
Abstract: This is the first report of micropropagation protocol for Chenopodium ambrosoides using wild grown plant materials. The development of this system gives the possibility of future reintroduction of this species, providing a way to stabilized or re-establish its population. The organogenic callus derived from nodal explants were put on Murshige and Shoog (MS) medium comprising 1.5 mg/l benzyladenine (BA) and 1.5/1.0 mg/l benzyl adenine/nephthalenaceitc acid (BA/NAA) formed multiple shoots. The highest shoots number (16.02±0.82) was formed at 1.5 mg/l BA in subculture. Under this condition some morphological anomalous characters as reddish and albino type shoots formation, narrow leaf lamina and long petiole emerged. The anomalies was controlled by mounting cultures on medium supplemented with BA/NAA (1.5/1.0 mg/l) and the maximum number of shoots per explants was documented (21.89±1.94) with normal shoots morphologically (green in appearance, broad leaf lamina and short petiole). The uppermost number of roots (12.32±0.58) and length (12.81±0.72) was documented at 1.0 & 1.5 mg/l NAA respectively. Rooted shoots were successfully acclimatized into pots.
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