PJB-2018-657
Topic: A study on analysis of pesticide residues in pollen and nectar samples by high performance liquid chromatography collected from different agricultural areas of Pakistan
Mahnoor Pervez
Abstract
ABSTRACT Presence of pesticides in pollen and nectar has risk toward honey bee (Apis mellifera) population. In 2016 to 2017 a field survey was conducted in different agricultural areas of Pakistan to collect pollens and nectars. In studied areas excessive pesticides used. Pollen traps were collection were installed at front of the hive and placed in orchards and crop areas. Nectar samples were collected by capillary and centrifuge method. The compounds were extracted and analyzed through HPLC technique by using suitable mobile phase. Among total 150 pollen samples 131 were positive. The most frequent residues were imidacloprid (14.6%), fipronil (8.6%) and thiamethoxam (7.3%). Among all 100 nectar samples 46 (46%) samples were positive and 54 (54%) were negative showed no pesticide residues. The most abundant pesticide in nectar was imidacloprid (11%), thiamethoxam (5%) and fipronil (5%). Residues of 8 different types of pesticides were found in samples. In pollen samples residual level of imidacloprid and thiamethoxam detected at a level 0.091-0.76 µg/kg and 0.055-0.78 µg/kg respectively while in nectar samples imidacloprid and thiamethoxam detected at a level 0.009-0.53µg/kg and 0.045-0.56µg/kg respectively. Key points: Pollen, nectar, Honey bee (A. mellifera) decline, HPLC analysis, Pesticide residue
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