PJB-2018-556
EVALUATION OF IN VITRO CYTOTOXIC POTENTIAL OF MARTYNIA ANNUA AGAINST HUMAN CANCER CELL LINES
Saba Muazzam
Abstract
The current study aimed to investigate in vitro cytotoxic potential of medicinally important plant Martynia annua Linn. The species is known for its purported health benefits and used as antiepileptic, antiseptic, in epilepsy, tuberculosis and inflammation. All parts of plant extracts (leaves, stem, fruit, flower and root) and sub-fractions of leaves are screened for cytotoxic potential against two cancer cell lines human cervical carcinoma cancer cell line (HeLa P-13) and apoptotic inducible cell line (LN-18) using a cell proliferation assay (MTT) by measuring the mitochondrial succinate dehydrogenase enzyme activity for the evaluation of metabolic activity of cultured cells. After the screening, leaves and flower extracts showed highest cytotoxic activity i.e., (45.65 µg/mL and 95.31 µg/mL respectively) against HeLa and (117.1 µg/mL and 155.9 µg/mL respectively) against LN18. Significant reduction of cell viability observed in both cell lines in a concentration dependent manner. Antiproliferative activity also retained in phenolic rich fractions of leaves and considered significantly cytotoxic to both cell lines. These results could justify the use of Martynia annua for exploring anticancer compounds present in this plant.
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