PJB-2018-551
Investigation of Screening, Identifying, And Culturing Conditions of Antagonistic Endophytes to Wheat Head Blight
Peigen Zhang
Abstract
Fusarium Head Blight (FHB) is a wheat disease caused by Fusarium graminearum. Its epidemic has been widely observed in the main wheat producing areas all over the world. Management of FHB using biological control strategies is a necessary requirement of sustainable agricultural development. In this study, in order to screen antagonistic bacteria for effects on FHB, 13 endophytes were isolated from the stems, leaves, spikes, and roots of wheat. Among them, 6 were found to be antagonistic to Fusarium graminearum and XS-2 had the best antagonistic effect. Physiological and biochemical analysis and 16S rDNA analysis revealed that XS-2 was Bacillus amyloliquefaciens. The antagonistic spectrum test displayed that XS-2 also had good antagonistic activity against cotton and fruit pathogens. Nutrition tests showed that the most suitable carbon source was glucose, and the most suitable nitrogen source was peptone. The optimum growth temperature was 28 ℃, and the ideal pH value was 7.4. Resistance tests showed that XS-2 had strong resistance to chloramphenicol and ampicillin sodium. This study indicates that XS-2 has a good antagonistic effect on Fusarium graminearum and lays a theoretical foundation for the application of the strain, which could be a valuable resource for the biological control of FHB.
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