PJB-2018-502
EFFECT OF 60Co-γ IRRADIATION ON GERMINATION, GROWTH AND PHYSIOLOGICAL CHARACTERISTICS OF ALFALFA
Shen xiaohui
Abstract
In order to study the effect of irradiation mutagenesis on germination, growth and physiology of alfalfa (Medicago sativa L), four alfalfa cultivars namely the i.e. Xunlu, the Longmu806, the Zhaodong and the Aohan were irradiated by 60Co-γ irradiation with five doses of 50 Gy, 150 Gy, 300 Gy, 450 Gy and 600 Gy respectively. The result showed that seed germination rate, shoot length, root length, fresh yield, plant height and plant survival rate increased under the dose from 0 to 150 Gy, but decreased when the dose was from 300 Gy to 600 Gy. However, the branch number of alfalfa was increased following the dosage increasing. Under the dose of 50 Gy, the stem-leaf ratio was higher than that of control, and it then decreased when the dose further increasing. It was also found that 60Co-γ irradiation treatments could promote the activity of three anti-oxidant enzyme including superoxide dismutase (SOD), peroxidase (POD) and hydrogen peroxidase (CAT). Its sensitivity to 60Co-γ irradiation treatment was POD>CAT>SOD. The malondialdehyde (MDA) content was varied with different cultivars. The comprehensive analysis showed that 60Co-γ irradiation with the doses of 50 Gy and 150 Gy were the ideal mutagenic treatments, as they had higher mutagenic efficiency, their mutant materials were more abundant, and therefore they could be considered as the reasonable mutation methods.
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