PJB-2018-452
Regeneration and Multiplication of Moringa oleifera by Seed Germination, Callus Induction and Direct Multiplication
Asfandyar Chaudhary
Abstract
Moringa oleifera, known as the miracle tree, is a plant of great importance. The plant is well known for its diverse medicinal and nutritional properties. The objective of the research is focused on tissue culture of Moringa oleifera through callus induction, direct multiplication as well as from seed culture. The study is also focused on optimization of sterilization protocol and media for the tissue culture processes. As expected, Moringa oleifera gave promising results using MS Media supplemented with different concentrations of Plant Growth Regulators such as IAA, NAA, BAP, Kinetin and 2,4-D. Seed initiation gave optimum results on MS Basal media. Direct multiplication of the plant was observed with optimum results on MS media supplemented with 0.1mg/L BAP. For callus induction, effects of Plant Growth Regulator and photoperiod were observed. In Dark conditions, maximum growth was seen on MS media supplemented with 2, 4-D (2mg/L), and that in light conditions, MS media with 2,4-D (2mg/L and 3mg/L) gave almost similar results. Initially, seed coats were removed inside the Laminar Flow Hood but this was affecting the sterilization negatively. Seed sterilization was then modified by removing seed coats and sterilizing them outside the Laminar Flow Hood. The seeds were then transferred to the LFH for further sterilization, and then cultured on the growth media. For the sterilization of the explant for direct multiplication and callus induction, firstly Ethanol was used which caused bleaching effect. Later, Ethanol was removed from the protocol, reducing the bleaching effect to zero, and sterilization was maximized.
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