PJB-2018-450
Evaluation of green synthesized silver nanoparticles as probable antibiotic agents
Muhammad Iqbal
Abstract
In this study rapid and simple approach was applied for synthesis of silver nanoparticles using H. rhamnoides aqueous leaf extract. The plant extract acts both as reducing agent as well as capping agent. Synthesized silver nanoparticles were characterized by using techniques such as UV-Vis spectrophotometer, X-ray diffraction (XRD) analysis, scanning electron microscope (SEM), and dynamic light scattering (DLS) measurement. The antibacterial analysis of the green synthesized AgNPs was investigated against human pathogenic Gram-positive (Staphylococcus aureus) and Gram-negative (Salmonella typhi) bacteria. Results obtained from characterization revealed that AgNPs were in size range of 34nm and amorphous crystalline in shape. AgNPs treatment (0.35mg/100ul) showed maximum inhibition zone (19 and 21 mm) against Salmonella typhi and Staphylococcus aureus. Green synthesis is fast, cost effective, non hazardous and environment friendly as compared to chemically synthesis counterpart. It takes only 10-15 min for the conversion of silver ions into silver nanoparticles at room temperature without addition of any toxic compounds. AgNPs can be used as source of antibiotics for the treatment of infectious diseases caused by bacteria.
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