PJB-2018-411
Qian-Yu Yang
Abstract
This study examined compatibility between different strains of mycorrhizal fungi and germination-stage Orchidaceae seeds under different culture conditions. Seven strains of fungi were isolated from four wild terrestrial orchid plants, and subsequently identified as Tulasnella spp. using ITS-rDNA sequence analysis. Symbiotic relationships were then established between seven Tulasnella spp, Cymbidium mannii and Cattleya hybrid (varieties YongKang and YongDian) under different oatmeal agar (OMA) concentrations. OMA at 2.0 g / L was the optimal concentration for symbiotic seed germination and establishment of mycorrhizal symbiosis with C. mannii, with a maximum germination rate of 92.05 ± 2.45 % (strain Tcs1). Meanwhile, 12.0 g / L OMA was the optimal concentration for seed germination in C. hybrid, with maximum germination rates of 87.13 ± 3.40 and 75.63 ± 5.41 % (Tcs1, varieties YongKang and YongDian, respectively). Strain Tcs1 effectively promoted seed germination and establishment of symbiosis with both C. mannii and C. hybrida at all concentrations of OMA, while strain Tsk1 promoted seed germination and symbiosis with C. mannii. Variance analysis revealed a highly significant correlation between seed germination and growth index (GI) values in different mycorrhiza fungi strains and under different concentrations of OMA. Thus, the most compatible mycorrhizal fungi of germination-stage Orchidaceae seeds and the optimal co-culture conditions were revealed. Based on these experimental results, the specificity between Tulasnella spp. and these two orchid species and two varieties were preliminarily discussed, providing a basis for further analysis of the molecular mechanism of symbiosis between orchid and mycorrhiza fungi.
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