PJB-2018-401
COMPARATIVE STUDY OF BIO-CULTURING OF FRESH WATER ALGAE, SPIROGYRA AND HYDRODICTYON
hamad ashraf
Abstract
The research was concerned with the optimization of cultural conditions for algal biomass production of two local filamentous fresh water species. Algal species such as Hydrodictyon reticulatum, and Spirogyra communis, were collected from fresh water bodies of Lahore. These species were then cultured in glass container having 1000ml of culturing media. For this different cultural media, A-Z media, Bristol’s media and Bristol’s soil media, were evaluated for biomass production. Among all these tested media, Hydrodictyon gave maximum biomass (18.6g/L) in Bristol media whereas, Spirogyra gave the biomass of 10.5g/L in Bristol soil media.. Then the effect of different Carbon sources (Lactose, Glucose, Cellulose and Starch) supplement in Bristol media were evaluated for biomass production. Among all the sources tested Cellulose at 1g/L was found to be significant for optimal mass production of (15.81g/1L) and (18.6 g/1L) of Spirogyra and 1lHydrodictyon respectively. Both algal species gave insignificant results in all other carbon sources. Then the effect of different nitrogen sources (Ammonium nitrate, Ammonium Sulphate, Ammonium Chloride, Potassium Nitrate and Urea) were tested for biomass production. Among all the sources tested urea at 0.1 g/L was found to be best for optimal growth (20.7g/1000ml) of Hydrodictyon as compared to (16.86g/1000ml) of Spirogyra. However, cellulose as carbon source and urea as nitrogen source was optimized for significant growth of both the algal species. The comparison between the growth rates of both specimens was evaluated and it was concluded that both the species gave maximum growth upto 15 days of incubation and then the growth start decreasing gradually. The statically parametric studies indicated that the volumetric growth of Hydrodictyon is significant in the selected media as compared to Spirogyra.
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