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Alternaria leaf spot disease of broccoli in Pakistan and management of the pathogen by leaf extract of Syzygium cumini

Arshad Javaid, Gul-I-Rayna Shahzad, Naureen Akhtar and Dildar Ahmed

Broccoli (Brassica oleracea L. var. italica Plenck) is comparatively a new vegetable crop in Pakistan. Recently, a fungal leaf spot disease was noticed on broccoli plants. In the present study, causal organism of broccoli leaf spot was isolated and identified on morphological as well as molecular bases. Molecular characterization of the pathogen was carried out by nucleotide sequence analysis of rDNA ITS region. Alternaria brassicae (Berck.) Sacc. was identified as causal organism that also fulfilled Koch’s pathogenicity postulates. Methanolic leaf extract (0, 1, 2, 3, 4, 5 mg mL-1) of Syzygium cumini (L.) Skeels was evaluated for its antifungal activity against the isolated fungal pathogen A. brassicae. Various extract concentrations reduced fungal biomass by 4–46% over control. GC-MS analysis of the extract showed seven compounds in the extract. Among these, 9-Octadecenoic acid (Z)-, methyl ester (28.18%), 9, 12-Octadecadienoic acid (Z,Z)-, methyl ester (25.94%), Hexadecanoic acid, methyl ester (18.04%), and Phytol (13.46%) were major compounds.

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