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An insight into in vitro and in vivo pharmacological activities of Pinus wallichiana

Nazish, Ibrar Khan, Khalid Khan, Sadiq Azam, Ikhwan Ahmad and Inam Ullah

The aim of the present study was to screen the test samples (Cr. Met. Ext and different fractions of stem, leaves and resin) of Pinus wallichiana for possible in-vitro and In vivo pharmacological activities. The aqueous fraction of leaves presented good antibacterial activity (63%) against Vancomycin Resistant Staphylococcus aureus (VRSA), Ethyl acetate (EtOAc) fraction of resin against Acinetobacter baumannii (60.8%) and Cr. Met. Ext of the stem against Proteus vulgaris (62%), S. aureus (61.5%) and Escherichia coli (60.3%). All of the test samples showed low or no activity against test fungal pathogens. The phytotoxic activity of test samples was moderate to low at 1000μg/ml. The results of haemagglutination indicated lack of phytoagglutinins in P. wallichiana. The antioxidant activity was good to moderate at 500μg/ml and the activity was concentration dependent. Good and significant insecticidal effect was recorded against the selected test insects. The Cr. Met. Exts were found safe at the tested dose and no lethality was observed in acute toxicity assay. The test samples presented significant decrease in the number of writhes after intervals of 30, 60 and 90 minutes in a dose dependent manner. The percent Gastro Intestinal Tract (GIT) motility observed in test group treated with different doses of the test samples was; 46.35 (stem), 49.93 (leaves) and 42.72% (resin) at 100mg/kg and same decreased was observed at 200 and 300mg/kg. The test samples of the selected plant also possess significant anti-pyretic effect after 1, 2 and 3 hours

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