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Metabolic mechanism of ascorbic acid in Actinidia chinensis var. chinensis

Ji-Yu Zhang, De-Lin Pan, Tao Wang, Gang Wang and Zhong-Ren Guo

Ascorbic acid (AsA) concentration was determined in Actinidia chinensis var. chinensis ‘Jinyang’ and ‘Hort16A’ during fruit development. AsAconcentration was higher at early stage of fruit development, and then declined with fruit development. The expressions of L-Galactose pathway genes were detected and the results showed GDP-D-mannose pyrophosphorylase 1(GMP1), GDP-D-mannose-3, 5-epimerase 1(GME1) and L-galactose dehydrogenase 1(GDH1) genes transcripts were declined with fruit development. Statistically significant correlations analysis results showed that there were significant positive correlation for GMP1, GME1 and GDH1 expressions with AsA concentration, and the expression level relations between each pair of GMP1, GME1, GDH1 are significant positive correlationin these two species, suggesting that GMP1, GME1 and GDH1 in L-Galactose pathway play important roles in AsA accumulation in A. chinensis var. chinensis. The trend of L-ascorbate oxidase 1 (AO1) gene transcript was corresponding with the AsA concentration, and there was a significant positive correlation between AO1 expression and AsAconcentrationin these two species. The expression of four Monodehydroascorbate reductase (MDHAR) genes and one Dehydroascorbate reductase (DHAR) gene were different between two A. chinensis var. chinensis varieties, predicting that DHAR or MDHAR genes might not key genes for AsA biosynthesis in A. chinensis var. chinensis.

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