PJB-2018-346
FLUORESCENCE MICROSCOPY SHOWS SELF-COMPATIBILITY EVIDENCE IN NEW APRICOT GENOTYPES
MOHAMMAD ZARRINBAL
Abstract
Self-incompatibility is believed to ber a common trait among apricot (Prunus armeniaca L.) cultivars of Irano-Caucasian ecogeographic group whereas European apricots are traditionally considered as self-compatible. In this study, self-(in)compatibility was evaluated for 21 Iranian apricot genotypes by monitoring the pollen tube development through style using fluorescence microscopy approach. Also, we determined the fruit set after self-pollination under field conditions during two successive years. Based on microscopic images, genotypes were considered as self-compatible when leastwise one pollen tube were attained by the ovary within 96 h after pollination in most of the studied pistils. In self-incompatible ones, pollen tube growth was blocked and terminative callose plugs were observed. This technique allowed for a reliable and rapid assessment of pollination outcome and thus, self-(in)compatibility status. Nearly in all cases, microscopic observations confirmed the results of final fruit set following self-pollination in the field. According to our data, in addition to reconfirming some European cultivars, self-compatibility was proved in new Iranian genotypes including ‘AD731’, ‘AD640’ and ‘AC103’ whereas, others were confirmed as self-incompatible.
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