PJB-2018-281
COMPARISON OF TWO DISTICT BETASATELLITES-ASSOCIATED COTTON LEAF CURL KOKHRAN VIRUS STRAINS INFECTION WITH THE LEVELS OF MICRO RNAS
Fazal Akbar
Abstract
MicroRNAs (miRNAs) are endogenous, non-coding, small RNAs that play an important role in regulating gene expression. The study described here was designed to investigate the effects of two distinct strains of Cotton leaf curl Kokhran virus (CLCuKoV), [the strain present in cotton pre-resistance breaking, CLCuKoV-Faisalabad (Fai) and the resistance breaking strain, CLCuKoV-Burewala (Bur)], and their cognate betasatellites Cotton leaf curl Multan betasatellite Burewala (CLCuMBBur) and Cotton leaf curl Multan betasatellite Multan (CLCuMBMul) respectively on the accumulation of developmental miRNAs to know common drifts between the two strains and their cognate betasatellites. CLCuKoV-Fai and CLCuKoV-Bur were inoculated into Nicotiana benthamiana plants alone and also in association with their cognate betasatellites. The results have shown that CLCuKoV-Bur and its associated CLCuMBBur are highly effective up-regulators of host miRNA expression, contrasting strongly with CLCuKoV-Fai associated with CLCuMBMul. Thus, each strain of CLCuKoV induced distinct pattern of response and betasatellites contributed significantly.
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