PJB-2018-226
INFLUENCES OF SHADING INTENSITY AND GENOTYPIC DIFFERENCE ON FLAVONOID AND POLYSACCHARIDE CONCENTRATIONS IN MICROPROPAGATED ANOECTOCHILUS PLANTETS
Changli Chen
Abstract
Anoectochilus plants are being extinct by the overexploitation for yielding bioactive compounds. In this study, micropropagated plantlets of A. roxburghii (Wall) Lindl., A. koshuaensis Hayata, A. formosanus Hayata and two newly found varieties (Fu_1, Fu_2, Tai, Fu_New_1 and Fu_New_2, respectively) were cultured under different shading treatments for seven months. The highest flavonoid concentration was found under the low shade intensity (2000 Lx). The ‘Fu_1’ and ‘Fu_2’ species had the highest rutin concentration under the low shade intensity, while the ‘Fu_New_2’ species had the highest polysaccharide concentration under the medium shade intensity (800 Lx). Concentrations of total flavonoids, kaempferol, quercetin, and isorhamnetin were also highest in the ‘Fu_New_2’ species. Therefore, the light intensity of 2000 Lx was recommended for flavonoid and polysaccharide from A. koshuaensis Hayata plantlets.
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