PJB-2018-196
Effects of arbuscular mycorrhizal fungi to the repair capacity of perennial ryegrass (Lolium perenne L.) in the uranium-containing soils
Lishan Rong
Abstract
Objective: This study is to research effects of arbuscular mycorrhizal fungi (AMF), including Glomusclaroideum (G. claroideum) and G. mosseae strains, to the repair capacity of perennial ryegrass (Lolium perenne L.) in the uranium-containing soils. Methods: The soil mixture was sterilized for three times at 120oC for 1 hour. The uranium (U3O8) was sprayed into the soil mixture, which was mixed well to obtain the uranium-containing soil mixture with 5 mg/kg U3O8. After one week, AMF was inoculated into every flowerpot and chamber device. After one more week, seeds of perennial ryegrass were planted with thirty seeds in each flowerpot or each chamber device. Perennial ryegrass plants were harvested 60 days after sowing in soils. Photosynthetic pigments, soluble proteins, and malondialdehyde were determined by ethanol extraction method, Coomassie brilliant blue method, and thiobarbituric acid method, respectively. Activities of alkaline phosphatase and succinate dehydrogenase were also determined. And, cellular and subcellular distributions of uranium in plant epidermal cells were observed with Hitachi H-7650 transmission electron microscope. Results: AMF influents photosynthetic pigment levels of perennial ryegrass. AMF increases the soluble protein levels in perennial ryegrass and represses the uranium-induced increase of malondialdehyde levels in perennial ryegrass. G. mosseaes has a more effective function than G. claroideum on the attenuation of uranium damages on cell structures of perennial ryegrass. Conclusion: Our results suggest that AMF can effectively increase the ability of perennial ryegrass on repairing the uranium-related soil contamination.
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