PJB-2018-87
IN-VITRO ANTIFUNGAL, ANTIBACTERIAL, PHYTOTOXIC, BRINE SHRIMP, INSECTICIDAL ACTIVITIES AND COMPOSITION OF THE ESSENTIAL OIL OF TAGETES MINUTA FROM DIR-KOHISTAN, PAKISTAN
Abdul Khaliq Jan
Abstract
Abstract The essential oil from the flowering shoot of Tagetes minuta L., collected from Dir Kohistan of Pakistan, was investigated for biological activities and chemical composition. The GC and GC/MS analysis data showed that among twenty two compounds, verbenone (25%), 1,1,1-trifluro-2-hexanone (11.6%),"4-(5-methyl-2-furanyl)-2-butanone"(8.8%) and D-limonene (8.4%) were the major constituents. The biological activity results showed that, out of six bacteria stains, the oil possessed inhibitory activities against S. flexenari and S. aureus with zone of inhibition 22mm and 20mm respectively. Among five fungal strains, only Aspergillus flavus and Candida albicans were susceptible to oil, with 60% and 30% inhibition respectively. In insecticidal tests the oil was noted for its effectiveness toward Sitophilus oryzae, with 50% mortality. Although the oil showed no cytotoxicity against Artemia Salina, while good phytotoxic activity was observed for Lemna minor. Key word index Essential oil; Tagetes minuta L.; verbenone; 1,1,1-trifluro-2-hexanone; antibacterial; antifungal cytotoxicity; phytotoxicity; insecticidal activities.
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