PJB-2018-44
EX-SITU CONSERVATION AND BIOCHEMICAL ANALYSIS OF EXOTIC CULTIVARS OF BANANA
ZAINY
Abstract
Present study evaluated impact of different (slow growth/storage) media (M1, M2, M3, M4, M5, M6, M7 and M8) on morphological and biochemical traits of three banana cultivars (Pisange, Brazillian and William) under two temperature (18°C and 26°C) treatments to develop an efficient protocol for short term ex situ conservation. The experiments were conducted at National Agriculture Research Centre (NARC) Islamabad during 2015. Analysis of Variance (ANOVA) showed great variations for almost all the morphological traits at different media and temperature however cultivars showed non-significant variations. Significantly higher growth (plant height) and number of shoots in all the cultivars recorded at 26°C, however number of roots at both the temperatures were non-significant with each other. Biochemical analysis of the cultivars after short-term conservation (5 months) showed significant variations at both temperature as well as media. Cultivar 2 (Brazillian) accumulated substantially higher amount of proline and soluble sugar contents as compared to other cultivars (Pisange, William) at both the temperatures. While chlorophyll a, b, and total chlorophyll content was significantly higher in cultivar 3 (William). Overall maximum proline and chlorophyll (a, b) contents were recorded in the cultures incubated at 26°C and 18°C respectively. Based on results of present study it may be concluded that 26°C is optimum temperature for maximum and rapid growth of shoots while medium (M2, M3) and temperature 18°C is most suitable for slow growth, maximum survival percentage (100%) with healthier plantlets (lush green) for ex situ conservation of banana cultivars (Pisange, Brazillian, William). Hence temperature 18°C in combination with M2 and M3 is recommended for short-term ex situ conservation of these exotic banana cultivars on the other hand for maximum growth temperature 26°C is best.
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